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ri-1(人B细胞淋巴瘤)

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Synonyms: ri-1
Background: The cell line Ri-1 has been established from the peripheral blood of a 48-year-old female patient with B-cell lymphoma. This EBV-negative cell line with 14q+ abnormality was shown to be at an intermediate stage of B-cell differentiation. Ri-1 expresses surface and cytoplasmic immunoglobulin (IgM kappa), HLA-DR, CD20, CD38 and FMC7. Rearranged heavy-light chain genes and one rearranged kappa light-chain gene was detected as well as a kappa and lambda light-chain gene in germ-line configuration.
Species: human (Homo sapiens)
Tissue: blood
Disease: Human Caucasian B-cell lymphoma
Gender: 48-year-old female
Morphology: Lymphoblastoid
Growth Mode: Suspension
Doubling Time: N/A
DNA Profile:  Cell Line Authentication Service
Culture Medium: RPMI-1640+10%FBS
We strongly suggest to purchase the complete medium
Cryopreservation medium: 90%FBS+10%DMSO
Karyotype: 92XXXX/69XXX,t(1,2)(p32,q34)t(2,8)(p11,q24)t(14,18
Subculture Routine: Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cell growth is very slow after resuscitation; large amounts of debris present.
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