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HRP anti-β-Amyloid, 1-40 Antibody (Previously

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Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Aqueous buffered solution containing a carrier protein and preservatives.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store between 2-8°C. Do not store antibody diluted below 50 ug/mL in the absence of protein (i.e.: add 2% bovine serum albumin).
Application

IHC-P - Quality tested
Dot Blot, ELISA, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 11A50-B10 is reactive to the C-terminus of beta amyloid and is specific for the isoform ending at the 40th amino acid.

Application References

(PubMed link indicates BioLegend citation)
  1. Watts JC, et al. 2014. Proc. Natl. Sci. USA 28:10323. (IHC-P) PubMed
  2. Diomede L, et al. 2014. Neurobiol. Dis. 62:521. (Dot Blot) PubMed
  3. Watanabe H, et al. 2012. J Neurosci. 15:5085. (ELISA) PubMed
  4. Portelius E, et al. 2006. J. Proteome Res. 4:1010. (IP)
  5. Vingtdeux V, et al. 2011. FASEB J. 25:219. (ELISA) PubMed
  6. Ohmi K, et al. 2011. PLoS One 11:e27461. (IHC-Other) PubMed
Product Citations
  1. Casali BT, et al. 2020. Neurobiol Dis. 142:104956. PubMed
  2. Casali BT, et al. 2018. J Neuroinflammation. 15:43. PubMed
  3. Cheng-Hathaway PJ, et al. 2018. Mol Neurodegener. 13:e0200060. PubMed
RRID
AB_2564979 (BioLegend Cat. No. 805407)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid, 1-40 on UniProt.org
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